Fans of Upton Park club have a history of taunting their London rivals.
West Ham have written to fans to warn them against making any ant-semitic chants during Wednesday’s game with Tottenham.
Past fixtures have seen Hammers fans taunt their Tottenham counterparts with offensive chants.
Spurs fans traditionally have strong links with London’s Jewish community, a heritage shared by West Ham chairmen David Sullivan and David Gold
“Any fan acting inappropriately – including racist, anti-Semitic and homophobic behaviour – will be banned from attending future matches,” Sullivan and Gold said in a statement.
“It is vitally important that every West Ham United supporter attending the fixture remembers that they are acting as ambassadors for our club and their behaviour should reflect the values and standards that we set,” the statement continued.
Gold has previously revealed that his great grandfather killed himself after suffering anti-Semitic taunts.
West Ham are currently sixth in the Premier League table, while Spurs are second, just two points behind league leaders Leicester.