High profile summer signings Arda Turan and Aleix Vidal are among those now eligible to play for Luis Enrique's side.
Barcelona have reportedly registered 77 new players across the club’s various teams as their Fifa-imposed transfer has finally ended.
In 2014, Barca were found to have breached Fifa regulations pertaining to transfers of youth players, with the one-year transfer ban being upheld on two separate occasions by the Court of Arbitration for Sport.
High profile summer signings Arda Turan and Aleix Vidal are the best known names on the list registered with the Catalan football federation on Monday.
Among those now able to play for the club’s youth teams are South Korean 17-year-old Seungwoo Lee, who was one of the non-Spanish youngsters involved in the Fifa investigation which led to the transfer ban. He will be officially registered when he turns 18 on Wednesday January 6.
Among the list of players are five new signings for the Barcelona B team – Jokin Ezkieta, Moi Delgado, Xemi, Dani Romera and 19-year-old Brazilian winger Robert Goncalves, who has come in on a loan deal from Fluminense which could lead to a €9 million permanent transfer next summer.
Daniel Gudjohnsen, son of former Barcelona and Iceland forward Eidur Gudjohnsen, is to join Barca’s Benjamin B side, having formerly played for the youth ranks of regional Catalan club Gava.
Luis Enrique, meanwhile, is still expected to sign at least one more player during the current transfer window, with former Manchester City midfielder Denis Suarez and Celta Vigo forward Nolito among those linked with a move to Camp Nou.