Chile coach Marcelo Bielsa has cut his 30-man squad down to 25 following their defeat to Mexico.
Bielsa had originally named a 30-man squad but it was cut to 25 after his men were beaten 1-0 in a Mexico City warm-up match on Sunday.
All five of the axed players are midfielders, with Manuel Iturra (Universidad de Chile), Pedro Morales (Dinamo Zagreb), Jaime Valdes(Atalanta) and Colo Colo pair Charles Aranguiz and Jose Pedro Fuenzalida all dropped from the line-up.
Iturra’s omission comes after he was red-carded for a foul on Andres Guardado just two minutes after coming off the bench in Sunday’s defeat.
Argentinian Bielsa will leave out two more players from his squad in time for the announcement of the final lists on 1st June.
Chile play their opening match of the World Cup finals in South Africa against Honduras on 16th June, and will also face Spain and Switzerland in Group H.