The English Football Association has lodged a written complaint with Uefa following racist abuse aimed at England Under-21 players during Tuesday’s 1-0 defeat in Spain.
Strikers Carlton Cole and Darren Bent were subjected to monkey chants during the first half, prompting the FA to issue a complaint.
“We will write to Uefa and Fifa about the jeering,” said an FA spokesman.
“We would, of course, hope that this does not occur again this evening,” said the FA’s head of media Adrian Bevington.
England under-21 coach Peter Taylor confirmed that he heard the abuse.
“I thought I heard something in the first half – it was disappointing,” said Taylor.
“It happened when Carlton was running through towards the Spanish goal.”
The actions of the fans come amid a revival of the controversy surrounding senior Spanish coach Luis Aragones and the remarks he made in October about Thierry Henry ahead of Spain’s game with France.
Aragones was heard shouting racist abuse, but he claimed he was trying to motivate Henry’s Arsenal team-mate Jose Reyes in training.
He has not made a personal apology to Henry, although the Spanish Football Federation apologised formally to Arsenal.
“I can’t say if it’s a reply to what I could have said or not but, in my opinion, racism is a question of conscience and I have my conscience clear,” Aragones said at a press conference on Tuesday.