Portugal have suffered a setback with the news that Nani has been ruled out of the World Cup finals through injury.
The Manchester United winger has been forced to miss the showpiece event in South Africa after picking up a shoulder injury in training last week.
“After tests we conclude he is unfit to participate in the World Cup,” read a statement from the Portuguese Football Federation.
“The medical file will now be presented to the medical department of Manchester United.
“In light of the report, the Portuguese Football Federation, taking into account the Fifa regulations for the 2010 World Cup, will call-up Ruben Amorim as a replacement.”
Portugal also have injury concerns over defender Pepe, who has been recovering from a knee injury he sustained in December.
The Real Madrid centre-back has not played since then but has been cleared by doctors from both his club and country to travel to the finals.
Portugal’s first match of the tournament is on 15 June against Ivory Coast in Group G, which also includes Brazil and North Korea.