Uefa has rejected appeals by Real Madrid and Lazio against punishments for the racist behaviour of some of their fans.
As a result, Lazio must play their next European tie behind closed doors and pay an additional fine of 32,300 euros.
It is the third time that Lazio have been penalised for race-related offences.
Real’s fine of 9,780 euros for racially abusing Bayer Leverkusen’s black players during the game at the Bernabeu on 23 November still stands.
Lazio’s ban will carry over until they next qualify for Europe.
Partizan were fined 5,200 euros after their fans threw flares during the 2-2 draw at the Stadio Olimpico.