lost their unbeaten record in 2010 World Cup qualifiers with a 2-1 defeat away to Colombia, with Carlos Tevez sent off after just 25 minutes.
Lionel Messi gave Argentina a first-half lead but Colombia responded after the break with a Ruben Dario Bustos free kick and an 83rd minute winner from substitute Dayro Moreno.
Argentina looked tired in the second half of a game played at 2,600 metres above sea level.
Neither side created a serious chance before Manchester United’s Tevez was sent off for kicking Bustos in an off-the-ball incident.
The dismissal appeared to inspire Argentina more than Colombia and the visitors went ahead 12 minutes later when Messi evaded three defenders before flicking the ball beyond Agustin Julio into the far corner.
Hugo Ibarra and Riquelme both missed chances to add to their score as Argentina controlled the ball for the rest of the first half, while Messi was close to a second in the 51st minute when he broke down the right, cut inside his marker but shot wide.
Ruben Dario Bustos drew Colombia level just past the hour mark with a brilliant free-kick and the home side grabbed the winner eight minutes from time as Dairo Moreno converted Freddy Grisales’s cross.